加入收藏
百奥莱博公司客服电话百奥莱博QQ百奥莱博QQ百奥莱博QQ
百奥莱博公司微信服务号

产品目录

LC3B/MAP1LC3B抗体图片
产品货号:
Z19901
中文名称:
LC3B/MAP1LC3B抗体
英文名称:
Anti-LC3B/MAP1LC3B antibody
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称Anti-LC3B/MAP1LC3B antibody
指标别名LC3;LC3B;MAP1A/1BLC3;MAP1A/MAP1B LC3 B;MAP1A/MAP1B light chain 3 B;MAP1ALC3;MAP1LC3B
克隆性Polyclonal
检验物种human,mouse,rat
应用范围WB,IHC,ICC/IF,IP,FCM(Intracellular),Direct ELISA
基因名称MAP1LC3B
抗体来源Rabbit
抗体类型IgG
免疫原E.coli-derived human LC3B/MAP1LC3B recombinant protein (Position:M1-G120).
实际分子量15~18KD
成分500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol.
纯化方式Immunogen affinity purified.
浓度500μg/mL
产品形态溶液
保存条件12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
背景资料LC3B,also named as MAP1LC3B,MAP1A/1BLC3,belongs to the MAP1 LC3 family.It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins,which are involved in microtubule assembly and important for neurogenesis.In cell biology,autophagy,or autophagocytosis,is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery.It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes.Two forms of LC3,called LC3-I (17~19kd) and -II(14~16kd),were produced post-translationally in various cells.LC3-I is cytosolic,whereas LC3-II is membrane bound.The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form,LC3-I.This is activated by APG7L/ATG7,transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form,LC3-II.The amount of LC3-II is correlated with the extent of autophagosome formation.LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes.MAP1LC3 has 3 isoforms MAP1LC3A,MAP1LC3B and MAP1LC3C.MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue,like their rat counterpart,MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form.This antibody is specific to LC3B.
Uniprot IDQ9GZQ8
推荐配套试剂Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P) and ICC.
推荐稀释比
Western blot (WB) 1:500~2000
Immunohistochemistry in paraffin section (IHC) 1:50~400
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50~400
ImmunoPrecipitation (IP) 1:50
Flow Cytometry(Intracellular) 1~3μg/1×106 cells
Direct ELISA 1:100~1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

相关搜索:LC3B/MAP1LC3B抗体LC3B抗体MAP1LC3B多抗Anti-LC3B/MAP1LC3B antibody
首页 |  关于我们 |  联系我们 |  在线咨询 |  网站地图
北京百奥莱博科技有限公司 京ICP备14007103号-3